Incredibly well-said by Talley Lambert and Jennifer Waters

Techniques for in vivo #serotonin detection in the brain: State of the art. #FreeAccess review: bit.ly/jnc15865 Westlake University PiatkevichLab Wiley Neuro

Our #chemigenetic #fluorescent calcium indicator WHaloCaMP is now out on #bioRxiv : biorxiv.org/content/10.110… WHaloCaMP can be used to report on neuronal activity in flies, zebrafish and mice. WHaloCaMP is great for #multiplexedimaging. A 🧵:

Next-generation green and red norepinephrine (NE) indicators are out in Nature Methods! Incredible team effort with Fellin Lab - Italian Institute of Technology J. Simon Wiegert A. Reiner Lab Johannes Bohacek Institute of Pharmacology and Toxicology. Grateful to our funders European Research Council (ERC) Swiss National Science Foundation DEEPER_project

Two mutations convert a natural fatty acyl-CoA synthetase from nonluminescent fruit fly into a luciferase (“FruitFire”) capable of generating light with the synthetic luciferin CycLuc2 in vivo (in the mouse brain). Not a de novo luciferase but shows how new functions can be

Researchers have developed Opto-RhoGEFs, an optimised optogenetic toolbox to reversibly control Rho GTPase activity, advancing the ability to manipulate the barrier between endothelial cells. elifesciences.org/articles/84364…

New jGCaMP8 viruses available Addgene !! Get 'em while they're hot! #GCaMP #neuroscience #calcium

Excited to introduce our optimized green and red fluorescent dopamine (DA) GRAB sensors #GRABSensors #dopamine Great collaboration with Mitsuko Uchida‘s group at Harvard University, Yingjie Zhu’s group at SIAT, John Williams’ group and Marina Wolf’s group at OHSU.

Nature research paper: A bioelectrical phase transition patterns the first vertebrate heartbeats go.nature.com/3RDdUcT

BREAKING NEWS The 2023 #NobelPrize in Physiology or Medicine has been awarded to Katalin Karikó and Drew Weissman for their discoveries concerning nucleoside base modifications that enabled the development of effective mRNA vaccines against COVID-19.

BREAKING NEWS The Royal Swedish Academy of Sciences has decided to award the 2023 #NobelPrize in Physics to Pierre Agostini, Ferenc Krausz and Anne L’Huillier “for experimental methods that generate attosecond pulses of light for the study of electron dynamics in matter.”

BREAKING NEWS The Royal Swedish Academy of Sciences has decided to award the 2023 #NobelPrize in Chemistry to Moungi G. Bawendi, Louis E. Brus and Alexei I. Ekimov “for the discovery and synthesis of quantum dots.”

I realize that almost all of my followers are GCaMPers, but another reminder that we're trying to work on climate stuff too. Would love to co-submit something on C capture, T tolerance, biofuel, plastic, anything.

Are you using the red dopamine (DA) sensor RdLight1 in combination with blue light for dual-color optogenetics/photometry? If so, read on... biorxiv.org/content/10.110… 1/n

This thread is an important reminder that all RFP-based biosensors could exhibit photoactivation under the right/wrong conditions. mApple is the worst offender, but the mechanism could be inherent to the chromophore of all RFPs-based biosensors. 🧵 1/5 doi.org/10.1021/cn4000…

Want to know what the burning questions are in brain energy metabolism research? Read vignettes on #lactate #citrate #glycogen #aspartateshuttle #spreading depression #ketones and more from experts in the field. doi.org/10.1111/jnc.16…

Here's a nice summary of our recent preprint on how to do 2P voltage imaging with FRET-opsin voltage indicators. It turns out you need to populate a photocycle intermediate state to see voltage sensitivity!

PSA: Spectra of Janelia Fluor dye-HaloTag conjugates are available at FPbase . Go to the spectral viewer and type in the dye of choice. fpbase.org/spectra/

Online now! AspSnFR: A genetically encoded biosensor for real-time monitoring of aspartate in live cells by Lars Hellweg, Kai Johnsson, Philipp Leippe, and colleagues at EPFL Max Planck Institute for Medical Research CeMM dlvr.it/T7Vyjf #chembiol

The first Microtutor interactive virtual imaging course is now live on the Global BioImaging training platform! Learn fundamental principles that are critical to rigorous fluorescence microscopy experiment design. Free & open to all! Funded by CZI Science. microtutor.globalbioimaging.org