A. Aktalay et al. report "Cleavable Linker Incorporation into a Synthetic Dye-Nanobody-Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy". Article open-access at …mistry-europe.onlinelibrary.wiley.com/doi/10.1002/cb…

New paper by L.M. Ostersehlt, D.C. Jans et al. in Nature Methods, from JakobsLab and Stefan Hell Labs. #MINFLUX #nanoscopy with multiplexed DNA-based labeling (DNA-PAINT) #microscopy #superresolution MPI for Multidisciplinary Sciences. Open-access at nature.com/articles/s4159…

MINSTED localizes fluorophores at Ångström precision. Check out the new article by M. Weber, H. von der Emde et al. in Nature Biotechnology. Open access at nature.com/articles/s4158… #Nanoscopy #Superresolution #Fluorescence #Microscopy MPI for Multidisciplinary Sciences

Interested in the raw data of our paper “MINSTED nanoscopy enters the Ångström localization range”? nature.com/articles/s4158… Here it is: doi.org/10.17617/3.0YV…

Published in Science: "MINFLUX dissects the unimpeded walking of kinesin-1" by Jan O. Wolff, Lukas Scheiderer, Tobias Engelhardt, Johann Engelhardt, Jessica Matthias and Stefan W. Hell. science.org/doi/10.1126/sc… Science Magazine Max Planck Institute for Medical Research #MINFLUX #Fluorescence #Microscopy

Intra-molecular resolution. With focused visible light: biorxiv.org/content/10.110… MPI for Multidisciplinary Sciences Max Planck Institute for Medical Research #MINFLUX #FRET #Fluorescence #Superresolution #Microscopy

It’s wonderful to see so many successful alumni and current lab members driving the field forward and making an enormous impact. I am very proud of all of you!

Published in PNAS: "4Pi MINFLUX arrangement maximizes spatio-temporal localization precision of fluorescence emitter" by J.D. Rickert, M.O. Held, J. Engelhardt & S.W. Hell. Check it out (open access) at pnas.org/doi/epdf/10.10… Max Planck Institute for Medical Research #MINFLUX #Fluorescence #Microscopy

Chemistry contributions – check them out if you haven’t already! A.N. Butkevich in Org. Lett.: pubs.acs.org/doi/10.1021/ac… K. Uno et al. in Org. Chem. Front.: pubs.rsc.org/en/content/art… D. Kim et al in JACS: pubs.acs.org/doi/10.1021/ja… #Fluorescence #Superresolution #Microscopy

Chemistry from the Hell Labs: D. Kim et al. in Chem. Methods: …mistry-europe.onlinelibrary.wiley.com/doi/full/10.10… A. Aktalay et al. in Angew. Chem. Int. Ed.: onlinelibrary.wiley.com/doi/full/10.10… E.A. Savicheva et al. in ChemPhotoChem: …mistry-europe.onlinelibrary.wiley.com/doi/full/10.10… A. Aktalay et al. in ACS Cent. Sci.: pubs.acs.org/doi/10.1021/ac…

Optical elements… with R. Orange-Kedem, Y. Shechtman et al. in Light Sci. & Appl.: nature.com/articles/s4137… J. Engelhardt, B. Ellerhoff, C.-M. Gürth, S.J. Sahl & S.W. Hell in J. Microscopy: onlinelibrary.wiley.com/doi/10.1111/jm… M. Leutenegger & S.W. Hell in Sensors: mdpi.com/1424-8220/22/2…

“Photoactivatable Large Stokes Shift Fluorophores for Multicolor Nanoscopy”. pubs.acs.org/doi/10.1021/ja… by I. Likhotkin, R. Lincoln, M.L. Bossi, A.N. Butkevich & S.W. Hell in JACS (2023). #Fluorescence #Dyes #Microscopy #Superresolution #Nanoscopy

Recent papers: G. Knorr, M.L. Bossi, A.N. Butkevich, S.W. Hell in Organic Letters (2024) pubs.acs.org/doi/10.1021/ac… M. Remmel et al. (2024) in Small Methods onlinelibrary.wiley.com/doi/10.1002/sm… M. Remmel, L. Scheiderer, A.N. Butkevich, M.L. Bossi,S.W. Hell in Small (2023) onlinelibrary.wiley.com/doi/full/10.10…

Nanoscopy, without the OFF… Check out the preprint by T.A. Hensel, J.O. Wirth & S.W. Hell (2024) “Diffraction minima resolve point scatterers at tiny fractions (1/80) of the wavelength” biorxiv.org/content/10.110… MPI for Multidisciplinary Sciences Max Planck Institute for Medical Research #Optics #Imaging #Microscopy #Superresolution

#MINFLUX tracking experiments in two color channels reveal a novel mode of kinesin stepping. Check out the recent preprint by L. Scheiderer, J.O. Wirth, M. Tarnawski & S.W. Hell “Dual-color MINFLUX: Kinesin-1 takes Chassé-Inchworm steps” biorxiv.org/content/10.110… Max Planck Institute for Medical Research

Published in Nature Methods (2024): L. Scheiderer, H. von der Emde, M. Hesselink, M. Weber & S.W. Hell "MINSTED tracking of single biomolecules" nature.com/articles/s4159… MPI for Multidisciplinary Sciences Max Planck Institute for Medical Research #Optics #Imaging #Microscopy #Fluorescence #Superresolution#Nanoscopy #MINFLUX #MINSTED

Published in Communications Biology (2024): J.O. Wirth, E.-M. Schentarra, L. Scheiderer, V. Macarrón-Palacios, M. Tarnawski & S.W. Hell “Uncovering kinesin dynamics in neurites with MINFLUX” nature.com/articles/s4200… Max Planck Institute for Medical Research #Microscopy #Superresolution#kinesin #protein #MINFLUX

#MINFLUX directly tracks CRISPR/Cas9-tagged endogenous dynein with nm/ms precision in living primary neurons, including direction reversals occurring rapidly at the time scale of single steps. Recent preprint by J.M. Schleske et al. biorxiv.org/content/10.110…

#MINFLUX fluorescence nanoscopy in biological tissue New preprint by T. Moosmayer, K.A. Kiszka et al. including PSD95 relative to the spine morphology (example below), presynaptic VGlut clustering and AMPAR clustering at the post-synapse. Check it out at biorxiv.org/content/10.110…

New article in Nature Communications by J.-E. Bredfeldt, J. Oracz, K.A. Kiszka et al. "Bleaching protection and axial sectioning in fluorescence nanoscopy through two-photon activation at 515 nm". Open access at nature.com/articles/s4146… MPI for Multidisciplinary Sciences #Optics #Imaging #Microscopy #STED