I just wrote a review on transcriptional regulation and I want to talk about something that has been bothering me for 5 years, which ended up changing how I think about science in general. A brief thread: authors.elsevier.com/sd/article/S09…

As I’ve gotten older, I’ve become more skeptical of any single study, regardless of how extensive the findings are supported in the paper. It’s impossible to be at the bench for as long as I have and not see how sensitive experiments are to 1000 different unknowable variables.

Our work on chemigenetic probes for photoacoustic imaging, in collaboration with Prevedel Lab is now published in J. Am. Chem. Soc. ! Congrats to Alexander Cook, Nikita Kaydanov and all co-authors!

Come be my colleague! Two faculty searches Whitehead Institute. One is focused on mammalian biology. The other on "emerging" biology including innovative new ideas or technologies, as well as artificial intelligence/machine learning. Apply today. wi.mit.edu/news/whitehead…

Brad Efron's 13 rules for giving a really bad talk (from efron.ckirby.su.domains/other/2013Thir…) Thanks to Robert Grant for finding this gem

From accidentally burning cells to hours of YouTube tutorials, my #FLIM #microscopy journey was a rollercoaster! Join me to learn what I discovered along the way. #AcademicChatter

Mike Fix

Parent tip: you can upload a photo of your kids printed paper school calendar to ChatGPT and ask it to generate an .ics calendar file that you can directly import

Happy that I could contribute to this story. Next step in plant #optogenetics. Probing plant signal processing optogenetically by two channelrhodopsins | Nature nature.com/articles/s4158…

#Rho #actomyosin folks, check out our latest work on the RhoA-specific GEF ARHGEF17. shorturl.at/NTZR8🧵

📢We are hiring a microscope specialist! Please apply and/or spread the word! hhmi.wd1.myworkdayjobs.com/en-US/External…

position alert! come do your PhD on predicting microbiome-immune system interactions with me: vacatures.uva.nl/UvA/job/PhD-Po…

SNAP-tag2: faster and brighter protein labeling biorxiv.org/content/10.110…

Joachim Goedhart Jonas Wietek  Addgene Jonas Wietek  Joachim Goedhart We've been testing it with some kinase translocation reporters. Very happy with the results so far! This channel shows the kinase reporter, cyan square indicates photoactivation of cells with varying levels of PdCO expression.

just for the pleasure of it, the fine choreography of epithelial cell migration with a PI3K mutation. This is the complex behavior we have to drug to treat cancer ... Movie from Lucien Hinderling . Switch sound on.

Joachim Goedhart It was discussed during the neuronal cytoskeleton webinar. 3 postdoc from 3 different labs and country ,we found that using Halo ligand over 100 nM and over 30 min incubation, you will see Halo ligand staining even in cells without tag. Vesicles filled with Halo.

ChatGPT is a game changer for me. Some years ago, I started learning programming for fun with the “simple” ImageJ macro language, by dissecting other people’s scripts (mostly jeromemutterer’s) and through trial and error. I loved it, and it became a real addiction! (1/7)

We present a phase separation-based reporter for AMPK activity and a proof of concept for a new dual reporter system that enables simultaneous monitoring of Ca2+ levels and AMPK activity. We hope the new tools will SPARK your interest!✨🔬 pubs.acs.org/doi/full/10.10… ACS Sensors

dear all, I got a brand new computer and consider installing windows and linux in dual boot ...particularly to get rid of some struggle with path and other issues with python. Any thoughts or recommendations ?

Tweeps, here's a question for you: Imagine you'd want to discriminate between E14.5 mouse kidney and E14.5 mouse testis by qRT-PCR. Which embryonic kidney-specific (i.e. much much lower or off in testis) or vice versa would you use/recommend?