Now on bioRxiv: Dari Kimanius paper on #Blush regularisation, a machine-learning based prior in #RELION5 trained on EMDB - EMPIAR @EBI entries, which he uses to solve a #cryoEM data set of a 40 kDa protein:RNA complex that was previously intractable! 🤯 biorxiv.org/content/10.110…

Writing successful grants in academia can be a challenge so I am v excited Florian Schur + Meytal Landau will be joining Itziar Serna Martin and I 2nd Nov 16-17 CET to discuss how they wrote their successful ERC Starter and Consolidator grants! Register here 👀⬇️tinyurl.com/ypezrx9u

1/ #CryoSPARC v4.4 is here! 🚀 Updates include tools to streamline repeat #cryoEM processing, reference based motion correction, orientation diagnostics, symmetry tools, improved 3DFlex, caching, data cleanup and more! cryosparc.com/updates

It was an amazing week of learning, discussions, and connection! 🌐 Best of luck to all our wonderful participants with their #cryoEM projects! Until next time! 🎓

Super proud of this paper now out on Nature Chemistry by Jon Machin nature.com/articles/s4155…. Great work Jon!! Post docs in our OMP team available- come and join us! postdocjobs.com/posting/7196207

Excited to share the results of a collaboration with CSL: Structural basis for inhibition of βFXIIa by garadacimab biorxiv.org/content/10.110… #garadacimab #antibody #cryoEM 🔬 A thread...🧵

This one has been a long time in the making… I’m really proud of a stunning paper (IMO!) but much more proud of a stunning team who made it possible Martin Wilkinson The Astbury Centre AstburyBiostructure RadfordLab Cell Press doi.org/10.1016/j.cell…

Happy to share our preprint on a tool that prototypes #cryoEM metadata harvesting! EMinsight analyses and reports on microscope configuration and experimental outcomes. We see potential applications in making data driven decisions, archiving, ML and more. doi.org/10.1101/2024.0…

Interested in membrane protein structure, folding, interactions and function? We're looking for a creative, talented post-doc to join our bacterial outer membrane team RadfordLab Neil Ranson in the The Astbury Centre, on a new Medical Research Council programme grant. jobs.leeds.ac.uk/Vacancy.aspx?r…

Just reviewed another paper on #cryoEM SPA. I have the impression that over-estimation of resolution is on the rise again. Often, poor maps are then "enhanced" with some post-processing neural network to hide some of the defects.

Our latest study in collaboration with Yogesh Kulathu lab describes the structure of UFM1 ligase complex bound to the 60S ribosome subunit. A heroic effort by Linda Makhlouf, Joshua Peter Joshua, Helge Magnussen and Rohan Thakur. Full details here: rdcu.be/dzff8

🔬 Join us for the Cryo-Electron Tomography virtual course from May 13-16, 2024. Hosted by University of Leeds, @eBIC_DLS, and University of Leicester this 4-day program is perfect for those new to the field. 🚀Follow the link rb.gy/7pw22m for more details! #cryoEM #cryoET

Excited to share our latest study reporting the structure and functions of the 55LCC ATPase complex. Free access below: authors.elsevier.com/c/1ir1ML7PXqQ76

High-res In-situ Structures of Mammalian Respiratory Supercomplexes in Reaction within Native Mitochondria biorxiv.org/content/10.110… Check out my lab's first work on membrane proteins! Yes, we knew nothing about detergent😜, so we directly imaged proteins within native membrane!

Since the outset of my career, I’ve always been fascinated by two types of dream research: (1) the intricate and dynamic biological systems, like the cellular transport machinery, and (2) the powerful imaging technologies that allow for visualization of the subtlest motions of

Interesting read, and I wonder how many projects would yield interesting observations with plunge freezing devices set to 37 °C nature.com/articles/s4158…

Interested in being introduced single particle cryoEM? Check out this Wellcome/MRC course and come visit us in Leeds!

The result of our very first intentional milling by Yehuda Halfon 🏳️‍🌈 and Joshua White with the new Hydra Bio PFIB-SEM on polished silicone! 😍📷 #FIBSEM #CryoEM

We are thrilled to share the first cryo-EM structure from the data collected during our Single Particle Wellcome/MRC course! Read it here: nature.com/articles/s4146… Congratulations to Victor Gawriljuk on this amazing achievement! It was wonderful to have you with us!

Very excited to share our paper on outer membrane assembly which includes the first cryoEM structures of BAM-SurA complexes. A great team effort with Jim Horne Joel Crossley Anton Calabrese RadfordLab Neil Ranson nature.com/articles/s4146…