***SUPER excited to announce an AMAZING speaker list for the 2025 Gordon Research Conference on Microproteins*** See more updates at: grc.org/decoding-micro… Be there or be square ⏹️ #microproteins #ORF #riboseq #cancer

High-quality peptide evidence for annotating non-canonical open reading frames as human proteins biorxiv.org/cgi/content/sh… #biorxiv_molbio

bioRxiv: High-quality peptide evidence for annotating non-canonical open reading frames as human proteins dlvr.it/TD2hl3

1/ Did you know the genome might have hidden protein-coding genes? In the 25 years since the Human Genome Project, research has been tied to a consensus set of 20,000 protein-coding genes. But the ‘dark genome’ has ‘dark proteins’ lurking in plain sight. biorxiv.org/content/10.110…

Interested in the "dark" proteome? Very excited to see this preprint out! One of the first of (hopefully) many new collaborations on the Ribo-seq ORF consortium. Check it out below! 👇

High-quality peptide evidence for annotating non-canonical open reading frames as human proteins biorxiv.org/content/10.110… --- #proteomics #prot-preprint

BREAKING NEWS The Human genome and proteome is larger than we thought. Human Proteome ISB

In the last 3 weeks I’ve learned that: The genetic code is … more of a guideline (via Slavov Laboratory) That proteins can be inherited (via Brent Derry) And now that not all proteins come from traditional genes (via Sebastiaan van Heesch) I think I need a lie down...

biorxiv.org/content/10.110… nature.com/articles/s4155… biorxiv.org/content/10.110… 🤯🤯🤯

1/ GENCODE continue to work on the annotation of non-canonical translations identified by Ribo-seq, and are pleased to support this preprint which examines which ORFs express protein products: biorxiv.org/content/10.110…

2/ Relatively few Ribo-seq ORFs have peptide support from tryptic digests, although substantial numbers are supported by immunopeptidomics data. Many thanks to our large number of collaborators Eric Deutsch Rob Moritz John Prensner Sebastiaan van Heesch

3/ How do we interpret immunopeptidomics support in biological terms? How many of these ORFs are bona fide proteins as opposed to unstable peptides or aberrant products? What does this mean for annotation? These are open questions and our work continues... HGNC UniProt

Sebastiaan van Heesch Very interesting work! Related to the deep learning question: We have this work where we look for micropeptides in ncRNAs. We detect the "noncoding RNAs" where the deep learning model insists that it is a protein-coding one. academic.oup.com/bioinformatics…

We're recruiting an experienced bioinformatician for our team! PrinsesMáximaCentrum van Heesch Lab. See below & apply if you're interested! werkenbijprinsesmaximacentrum.nl/en/vacancy/sen…