Many steps leading up to plant transformation, including storage and growth conditions of Agrobacterium, can affect the final outcome of your experiment. Seven speakers will guide attendees through their personal experiences working with Agrobacterium in Episode 2.

Thanks to MSU for the opportunity for Mr. Simpson #ECSU. So happy that he is having a fantastic time 🍚🍓. #VikingPride 🔵🔵🔵 #ECSUVikingsScience #hbcu

HortGenome Search Engine aids efficient gene and functional information querying across 500 horticultural crops. #Genomics #Horticulture #Bioinformatics Oxford Journals Details:doi.org/10.1093/hr/uha…

And with this last RUBY expression experiment, I can confidently say that pCambia2300-RUBYGREEN is ready for use!!! I just listed the plasmid in my webstore for the low price of $20. Making fresh stabs as we speak. Go support my lab and buy one today! atinygreencell.com/products/pcamb…

Hello World 🔴 #ECSU. @atinygreencell. 'Alice' petunia leaves 🍃 after Agro transformation 🧬🧪🦠🧫 with GV3101 + RUBYRED 🔴 at 2 weeks. Faster than my tobacco cultivars. 🤞🤞🤞 for the next 4 weeks. #ECSUVikingsScience #hbcu #new

This "frugal CRISPR kit" costs about $2. It includes a cell-free extract (no living cells), plasmids encoding colorful proteins, and Cas9 + guide RNAs. Students express colorful proteins in the extract & then use CRISPR to cut the protein-coding genes, leading to loss of color.

For anyone wanting to make cheap homemade DNA storage cards similar to "FTA" DNA storage cards by Whatman, here are two very similar formulations that have been tested and used for plants. Both could also probably be adapted for use with a wide range of other sample types: 👇 1️⃣

The Zea mays PeptideAtlas: A New Maize Community Resource | Journal of Proteome Research pubs.acs.org/doi/10.1021/ac…

Seeing the invisible: tools to teach and study plant transcriptional responses (Bikash Baral, Saku Riihelä, Jasmin Kemppinen, Maija Sierla, Mikael Brosché) buff.ly/3X2Ypgw ASPB #PlantSci

Yippee 🎆. Got these auxotrophic Agrobacterium from Intact Genomics, Inc. #ECSU. Should allow minimal or no Agro overgrowth on our plant tissues (saving money/time 👍🏼👍🏼). Undergrad project to transform 🧬🧪🧫 using most used plasmids 🔴🔵🟣. #ECSUVikingsScience #hbcu

Rooting of the first 'Baby with the Red 🔴 Gene' from Alice #ECSU. Sebastian S. Cocioba🪄🌷. First noticeable plant with red leaves from petunia. Transformed from GV3101 +RUBYRED 🧬🧪🧫🌱. Meristemoids completely red are growing much slower. #ECSUVikingsScience #hbcu

Finally out! A long journey w/ many setbacks over a decade. Frustrating growth variation of hiSA transgenics led to a COOL find of SA repression of COR genes. Ectopic expression of CORs can override SA inhibition of growth w/o affecting disease resistance. doi.org/10.1093/plcell…

Thanks to Robert C. #undergradresearcher for assisting me in an experiment on Agrobacterium 🧬🧪🧫 🔵🟣 transformation of petunia #ECSU. These PI hands ✋️ can no longer easily use forceps to plate more than 200 leaf 🍃 explants #GenX 🤣. #ECSUVikingsScience #hbcu

Welcome, Mr. Michael H. as our Research Associate #ECSU. He will be assisting in lab preps etc. ⚗️🧪🔬🐁🪴🐞. He is a graduate of both our BS (2020) and MS Biology program (2022; sensors in detection of COVID-19 spike 👑 proteins) #VikingPride 🔵🔵🔵. #ECSUVikingsScience #hbcu

Peer Mentoring 👍🏼 #ECSU. New #undergradresearcher Jayla under the watchful eyes of senior Juelle. Making and pouring her plant MS media 🧫. Btw, I was arranging my CDs 💿 😂. Conversations about 🎶 and 🎬 of the 80's and 90s. Can't lose the recipes. #ECSUVikingsScience #hbcu

Happy to be part of this endeavor👍🏼👍🏼. Fingers crossed 🤞🤞 that funds are allocated for these Plant Transformation Facilities for bioenergy crops U.S. Department of Energy . osti.gov/biblio/2335710/

Upcoming Event: Industry Career Panel! Join us on September 18th at 12 PM ET. Learn more and register at the PlantGENE website: plantgene.sivb.org/industry-caree…

Alice's 'Baby with the Red 🔴 Gene' has rooted #ECSU. @atinygreencell. Will place leaves 🍃 of this transgenic petunia plant back into PTC before acclimating. Still have several explants with red meristemoids, but no completely 🔴 shoots as yet. #ECSUVikingsScience #hbcu

Interested in #maize research? Our ongoing Maize collection at CSH Protocols is rapidly growing and is being updated every day! Check out the latest articles (link below)⬇️

Busy weeks for General Botany #ECSU. Wet lab 🧪⚗️ experiments have started. Colorful 🎨 macromolecule tests (+ water as a control; and an unknown soln). Also placed red onion 🌰 epidermal cells in glucose 🍬 or salt 🧂. Lab groups are gelling. #ECSUVikingsScience #hbcu