Twitter Beğeni Hilesi

Excited to share this discovery: Huntington's disease is a DNA process for almost all of a cell's life. Inherited HD alleles are innocuous, just unstable – CAG repeats slowy expand throughout life, acquiring toxicity only when quite long (>150 CAGs). biorxiv.org/content/10.110…

I am excited to share that our paper “Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9” from Doudna Lab is out in Cell! authors.elsevier.com/sd/article/S00…

Now online at Cell: “Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9”: sciencedirect.com/science/articl… . From authors Amy R Eggers, Kai Chen, Kasia Soczek, Owen Tuck, Erin Doherty, Bryant Xu, Marena Trinidad, Brittney Thornton, and Peter Hyungjun Yoon

My postdoc work in the Daniel Bauer lab is out Nature Biotechnology! We found that the limited nucleotide pool in hematopoietic stem and progenitor cells (HSPCs) constrains reverse transcription and thus prime editing (PE). nature.com/articles/s4158…

Excited to share our latest work, motivated by the challenge of efficient prime editing in HSCs. We find that the metabolic state of quiescent cells presents a hurdle—but it’s surmountable, by boosting dNTPs. Led by the incomparable Sébastien Levesque. rdcu.be/dJdgK

PE7 is hot! Great little feature of our work by Addgene. blog.addgene.org/hot-plasmids-s…

Deadline E X T E N D E D! Applications📄 are now⏳ being accepted for the 2024 MolBio Scholars program. For more info and to apply, visit: molbio.princeton.edu/graduate/molbi…

Congrats🥂 to Asst Prof Ai Ing Lim, a 2024 Searle Scholar for her lab's work toward understanding maternal-offspring immune crosstalk, that is, how the immune systems of moms and their offspring inform and tolerate each other! molbio.princeton.edu/news/2024/lim-…

What if we could universally recombine, insert, delete, or invert any two pieces of DNA? In back-to-back nature papers, we report the discovery of bridge RNAs and 3 atomic structures of the first natural RNA-guided recombinase - a new mechanism for programmable genome design

Introducing CHARM: a new epigenome editor to methylate DNA at the promoter of a targeted gene. Our lab's collaboration with Jonathan Weissman's Lab's Edwin Neumann & Tessa Bertozzi shows deep silencing of brain PrP Paper: science.org/doi/10.1126/sc… Blog: cureffi.org/2024/06/27/int…

This. is. NOT. yet "another" gene therapy paper. The patient's name is Michael, he is Terry Pirovolakis's son, and if you google that name and learn what it took to make this happen (watch a talk by Terry) - you'll read this paper in an entirely new light.

Check out our latest paper showing that HLTF removes Cas9 from DNA, explaining phenotypic differences when using the two Cas9 nickase variants. Important for gene editing! Thanks to Giordano Reginato & Rosaria and Martin Jinek Taekjip Ha andre nussenzweig lab nature.com/articles/s4146…

Today in Nature Biotechnology, we describe first-gen click editors (CEs), which couple the advantageous properties of DNA-dependent DNA polymerases and HUH endonucleases with RNA-programmable nickases for precise and versatile human genome editing. 🧬 nature.com/articles/s4158…

Thrilled to see our click editing paper out today in Nature Biotechnology! 🚀 Click Editors (CEs) are a new class of genome writers which combine DNA polymerases and HUH endonucleases with RNA-programmable nickases for precise edits without DNA DSBs🧬✨ nature.com/articles/s4158…

Is anyone interested in coming to work with the amazing crowd at the Cuella Martin lab and our cool projects? An RA position will open soon, stay tuned!

The Dept. of Molecular Biology at Princeton University invites applications for a tenure-track faculty position at the Assistant Professor level. We seek a colleague who will build a world-class research program in cell biology. For details and to apply: princeton.edu/acad-positions…

Can we recreate the transcriptional states seen in single-cell atlas projects? In our new preprint we try to do this using large-scale Perturb-seq experiments. Along the way we discuss off-target effects of CRISPR epigenetic editors and identify drivers of key fibroblast states.

No one can take apart a complex dataset like Thomas Norman!!!

Chromatin scientists, want to reorganize genomic loci as you wish? Out today in Cell--We made a tool that uses surface tension of engineered condensates to reposition DNA loci across microns of space in living cells in just a few minutes: VECTOR cell.com/cell/abstract/…

Thrilled to share my lab’s latest work, out recently in Mol Cell, on a role for HLTF in G4 resolution. Led by the amazing Gongshi Bai with essential contributions from @davidcortez, Brandt Eichman, Cejka Lab and The Boulton Lab. See: authors.elsevier.com/a/1jbBg3vVUPRm…