'We all know, intuitively, that Mike Johnson has a freezer full of human heads in his basement. But it's not been something that's easily provable.'

Congratulations to Qingyu Luo and our amazing team and collaborators! Tweetorial with more details to come soon... nature.com/articles/s4158…

We preset an approach to test phosphorylation site function in high throughput. Like phenotypic CRISPR screens but instead of knocking out a gene, we mutate specific codons with base editors so the resultant protein cannot get phosphorylated that site.

Modular vector assembly enables rapid assessment of emerging #CRISPR technologies
Abby V. McGee Broad Institute et al.
hubs.li/Q02v7f4V0
Up-and-coming genomics research from Cell Genomics

At long last, the in4mer paper is published! In4mer is our CRISPR/Cas12a platform, developed in collaboration with John Doench, where we show that expressing 4mer arrays of Cas12a guides turns out to be a highly efficient way to knock out genes singly or in combination.

I’m seeing some “free market” simps defending Nature’s 12k open access fees. Guess it’s time to bring this back around.

This is a very cool screen!

Splicing. It matters!