A fantastically focused effort by the lab spearheaded by Lukas Grätz and Maria Kowalski-Jahn to understands how FZD dynamics define the signaling outcome. nature.com/articles/s4146…

Another amazing paper on cAMP localization from Nina Tsvetanova! nature.com/articles/s4158….

Join our new lab at #Stanford as computational biologist/biostatistician to develop methods for analyzing, integrating, and visualizing quantitative proteomics data: careersearch.stanford.edu/jobs/senior-bi…. Please RT!
#TeamMassSpec

Check out this amazing paper from my UC San Francisco colleague Roshanak Irranejad demonstrating the importance of cAMP location in cardiac function. Impressive study spanning cell biology to physiology in two model organisms! nature.com/articles/s4158…

My first preprint about work at Schrödinger:
chemrxiv.org/engage/chemrxi…

We predict ligand efficacy via absolute binding free energy perturbation on different receptor conformations, confirming the thermodynamic link between ligand binding and the receptor's functional response.

Lab (recent) alumnus Alex Vizurraga Ph.D. is the Mol Pharm (ASPET) highlighted trainee for July! His first author paper detailing the discovery of a new GPR56 activating compound was published in the same issue.
aspet.org/aspet/news/new….

molpharm.aspetjournals.org/content/104/1/…

microscopy vs ultramicroscopy

CaaX-motif adjacent residues control G protein prenylation under suboptimal conditions biorxiv.org/cgi/content/sh… #bioRxiv

In our latest preprint we quantify template bias in reconstructions from high resolution template matching in vitro and in vivo. We show that using baited reconstruction allows us to get reliable high resolution reconstructions from 2D images in SPA and in situ.

I am seeking advice regarding the decision on an electronic notebook that we will be using in the lab: Which electronic notebook are you using and what do you like about it? #newPI

Do you love #computing , building #HPC systems, #algorithm development, and working in an academic environment with an active, diverse research team? The #cryoEM research center UW–Madison Biochem is recruiting a HPC administrator! Please RT and DM if interested! jobs.wisc.edu/jobs/high-perf…

I did something... I wrote about my experience surviving and ultimately thriving as a tenure-track faculty member with #bipolar disorder and #adhd for nature. If you see yourself in me, you are not alone... nature.com/articles/d4158…

An end of workshop announcement 📣!! We are launching a heterogeneity challenge for #cryoem ! Check out the details here: simonsfoundation.org/heterogeneity-… Download the datasets and upload your results by Sept 1!

Happy to share structure of human TRPV4 in complex with the GTPase RhoA. TRPV4-RhoA contact interface reveals residues that are mutated in neuropathies, providing insight into disease pathogenesis. Great collaboration with the lab of David Zhang (MCW). disq.us/t/4h6p0om

I recall a test someone did in the past showing classified particles from different cryo-EM processing software packages being different, but leading to similar results. Who, where? Twitter? CCPEM? 3DEM?

Searching for women speakers working on the cutting-edge of GRCRs & G protein signaling - look no further! Check out the #GPCRladies website list of PIs, Fellows and Postdocs. Thanks for organizing Silvia Sposini TasteLabHUJI, Moran David! DrGPCR
gpcrladies.com/about/

In the final manuscript submitted before summer holidays, we check how well the different all-atom force fields capture the effects of cholesterol on phosphatidylcholine bilayers.

chemrxiv.org/engage/chemrxi…

Please share with talented cancer biologists! The Department of Pharmacology, Physiology & Biophysics at Chobanian and Avedisian School of Medicine at Boston University is looking to hire a new assistant/associate professor in cancer biology. Link below:

academicjobsonline.org/ajo/jobs/25003

This protocols paper could be useful for those measuring #GPCR signaling with BRET biosensors made in our lab not long ago (or maybe other BRET systems)

onlinelibrary.wiley.com/share/author/Y…

The plasmids for the biosensors: addgene.org/browse/article…

So excited to share our new publication in Nature Immunology! We leveraged new bioinformatics pipelines and a synthetic biology approach to identify a novel GPCR-Gs-PKA immune checkpoint promoting T cell exhaustion and cancer immunology failure. nature.com/articles/s4159… Eytan Ruppin, MD, PhD