The next NUTechScan campaign! It is our pleasure to host the Deepcell REM-I in the Newcastle FCCF development lab space. Looking forward to classifying cells using label-free #machinelearning and #deeplearning features #cytometryaunatural #singlecellanalysis #flowcytometry

OPTIMAL: An OPTimized Imaging Mass cytometry AnaLysis framework for benchmarking segmentation and data exploration - Hunter - Cytometry Part A - Wiley Online Library onlinelibrary.wiley.com/doi/10.1002/cy… Really happy to see this out! @NUBiosciences Newcastle: Innovation, Methodology & Application NUTechScan #multiplexedimaging #imc

Great work by Newcastle FCCF showing how multiplexed analysis of tissue (by IMC) in this case, needs some proper checks and balances. #singlecellanalysis

Very excited for the Newcastle FCCF to be hosting the Cytek Biosciences #imagingflowcytometry day with our Amnis showcase. A great line of up speakers and subjects. Please let us know if you would like to attend @NUBiosciences #flowcytometry #singlecellanalysis Newcastle: Innovation, Methodology & Application NUTechScan

Great looking instrument. Very excited to see it in action soon! #singlecellanalysis #flowcytometry #imagingflowcytometry #morphometrics

Really enjoyed giving a talk for Miltenyi Biotec at ISAC in Montreal all about the MACSima highly multiplexed tissue imaging platform. #cyto2023 #singlecellanalysis #flowcytometry #spatialbiology

Really looking forward to next weeks Newcastle: Innovation, Methodology & Application NUTechScan seminar from the wonderful people @Deepcell_Inc Come and hear all about how label-free cell classification and sorting can be achieved from a bright-field image and #AI

So good to be able to announce this officially. Thanks to @NUBiosciences Joris Veltman and tim robson for the support. This is indeed a significant first for UK #technicians and I hope will break the glass ceiling for technical roles in the UK.

Are you looking to have a say in the UK's new Institute for Technical Skills and Strategy? We are looking for fresh voices, new perspectives and a willingness to shape the national landscape for technicians.

Apply for the Technical Council: forms.microsoft.com/e/bagtCcjjeX

We are close to announcing our next NUTechScan campaign after the incredible success of our first one! Watch this space..........@NUBiosciences Newcastle: Innovation, Methodology & Application #singlecellanalysis #flowcytometry

In conclusion, OPTIMAL is not a new analysis pipeline per se for IMC or any other multi-plexed tissue imaging data, but it does provide a simple framework for assessing any approach. As we convert segmentation data to .FCS file format, it also makes onward analysis accessible.

We also looked at data transformation using different arcsinh cofactor values and used the Fisher Discrimination Ratio, also know as Linear Discriminate Analysis, to determine a value of 1 gave the best statistical separation between low and high parameter values.

OPTIMAL: An OPTimsed Imaging Mass cytometry AnaLysis framework for Segmentation and Data Exploration biorxiv.org/content/10.110… Great to see this out finally. Standard BioTools #ImagingMassCytometry #singlecellanalysis

After the first very successful NUTechScan campagin to evaluate and now secure the Miltenyi Biotec MACSima system....what is next on the horizon! Watch this space.......

First grant success for NUTechScan ! Awarded ~£1 million from the Medical Research Council mid-level equipment call. Bids led by Andy Filby of Newcastle FCCF Dr Kelly Coffey and Alex Laude of Bio-Imaging Unit-Newcastle University and supported by many others Newcastle University @NUBiosciences shout out to FMSgrantfunding

Great trip to San Jose to attend the @DeepCell_Inc scientific summit. Heading back to Newcastle University and Newcastle: Innovation, Methodology & Application and NUTechScan with a head full of ideas about how we can use their cool tech to address novel questions and revist old #flowcytometry #morphometrics

One is a marvel of German engineering and design...the other is a BMW ;-) Our first NUTechScan campaign is about to begin with Miltenyi Biotec #spatial #singlecellanalysis non #flowcytometry ;-) Newcastle FCCF Bio-Imaging Unit-Newcastle University

Super second NUTechScan Newcastle: Innovation, Methodology & Application seminar this morning from Akoya Biosciences all about their PhenoCycler Fusion system.

Great first Newcastle: Innovation, Methodology & Application NUTechScan seminar yesterday from NanoString discussing their spatial analysis platforms, including the new COSMX #multiomics system. Such an exciting time to be involved in #horizonscanning for new technologies in the biomedical space!

Not one, but TWO exciting roles with the world-leading Bio-Imaging Unit-Newcastle University First role is for a dedicated #bioimaging #datanalyst tinyurl.com/2p9h57df and second one to support the wonderful work of the QUAREP-LiMi global initiative tinyurl.com/5n6bn7cy #singlecellanalysis