🧬The Genome Organization & Nuclear Function Meeting (CSHL Meetings) kicks off today!

🧑‍🔬Our Structural Genomics Group Leader, Marc A. Marti-Renom, is 1⃣ of the discussion leaders, presenting:

🗣️'Sex-determining 3D regulatory hubs revealed by genome spatial auto-correlation analysis'

Finally, we took a new lab picture with our newest members!

It was such as treat to have Wu Lab present at UNMC Dept of Genetics, Cell Biology & Anatomy, hear fascinating stories about the evolution of discoveries about homolog-pairing, and exchange deeply thought-provoking ideas. Thanks for your time. We had so much fun!!

Multiplexing with Exchange-PAINT can be also directly leveraged for tokPAINT as well as multi-omic cryosection protocols targeting proteins, DNA and RNA. [6/11]

Don't miss out on this important conversation! We hope you join us! Go to siesociety.org/events to register for free.

Great news, this does not only work in fixed cells but also in ultrathin cryosections of tissues, as we show for tokPAINT imaging of Pol II within mouse tissues. [5/11]

Looking forward to my first time giving a talk in front of a biophysics audience Biophysical Society today.
Come to ballroom B at 9:00 to see how I use chromosome walking in super-res to understand homologous chromosome communication. #BPS2024

Achieving sub-3 nm localization precisions, tokPAINT enables detection of Pol II down to level of single antibodies. Bonus: cryosections are openly accessible to probe diffusion, enhancing imager binding kinetics, e.g. for molecular counting via qPAINT. [4/11]

Inspired by previous work (e.g., by Prof Ana Pombo, Eric Betzig, HeilemannLab) we adopted Tokuyasu cryosectioning, widely known from Immunogold EM, to enable nuclear tokPAINT imaging of Pol II using TIRF. [3/11]

TIRF is an excellent imaging modality for DNA-PAINT: low background, high resolution, small imaging volume! Beautiful for DNA origami, ideal for cellular targets close to the cover glass. The drawback: its limited axial range. [2/11]

We are excited to share the latest preprint from the lab! Check out the story in the thread below from the lead author Johannes Stein

Cryosectioning-enabled super-resolution microscopy for studying nuclear architecture at the single protein level biorxiv.org/cgi/content/sh… #bioRxiv

We've teamed up with the SIE Society for a panel about the #science of #genetic traits, the social impact of on-screen stories, and their combined influence on our ideas about race. Join us! 2/15, 11 am-12 pm PT tinyurl.com/43fa768c

We are excited to announce our acceptance as an Interfaith America Fellow for IA's 2024 Faith & Health cohort! Learn more about PGED's project by reading Marnie's description on the announcement page: bit.ly/3ub2ZxI. #InterfaithAmerica #education #religiousdiversity

We wish you a fantastic start, Jumana! We can't wait to see all the cool science from your lab 🔬

#Geneediting can be used to reduce the toxicity of crops, save endangered species, & create hybrid organisms w/ traits from extinct species. Explore the potential risks & benefits of altering the environment in pged.org/lesson-plans/#…. #educators #curriculum #Genetics #bioethics

Check out the upcoming Cold Spring Harbor meeting on Genome Organization & Nuclear Function, Apr 20 – May 4, 2024, on advances in spatial and temporal aspects of nuclear structure & function. We look forward to seeing you! Wickstrom-Lab Jane Skok Andrew Belmont CSHL Meetings

Hey #transcription #MOET2023 #CSHL MOET2023 #CSHL peeps…
Looking forward to the speaker lineup on mechanisms of transcription starting tonight!
I’m presenting a poster Wednesday evening #71 and discussing how homologous chromosomes interactions could help gene regulation/txn

On my way to Vienna.. delayed flight from Milan 🥲 but I cannot wait to be at Single Molecule Localization Microscopy Symposium !! Looking forward for the exciting talks and discussions #SMLMS2023 #3Dgenome #genomics 🖥️🧬🐠🐟🔬

Congratulations, Laura Breimann!